TīmeklisMutantsDb is a database of mutants from R. solanacearum strain GMI1000 generated through random insertion of the transposon EZ-Tn5? from EPICENTER biotechnologies. TīmeklisThe EZ-Tn5™ Tnp Transposome™ is the stable complex formed between the EZ-Tn5 Transposase enzyme and the EZ-Tn5 Transposon. The EZ-Tn5 …
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Tīmeklis2024. gada 4. nov. · EZ-Tn5 Mutagenesis and Screening. Random mutagenesis was performed by electroporation of P. oryziphila 1257 competent cell with 1 μL of the EZ-Tn5 < R6Kγori/KAN-2 > Tnp transposome. The electroporated cells were immediately recovered with LB medium to the electroporation cuvette to 1-mL final volume. The … TīmeklisPK Ad V ¡ ¤³Ó öõ att_349260_1.pdfUT *x7d*x7dux é é ŒüsteϺ/ ' Û¶“Ží¬ŽmÛIǶmÛîtlÛ¶mÛNÞ ÷>÷ÞsÆØ¿wœgÔ˜…YõðSOÍúc-29! induction venturi
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TīmeklisRandom transposon mutations were generated with an EZ-Tn5™ insertion kit (Epicenter) in accordance with the manufacturer’s instructions. The 25D7 fosmid and transposon reaction mixture were incubated at 37 °C for 4 h. The reaction was terminated by adding 1 μL of stop solution. Then, the mixture was incubated at 70 °C … TīmeklisEZ-Tn5™ 10X Reaction Buffer 100 µl 0.50 M Tris-acetate (pH 7.5), 1.5 M potassium acetate, 100 mM magnesium acetate, and 40 mM spermidine. EZ-Tn5™ 10X Stop Solution: 100 µl 1% SDS solution. T7/KAN-2 FP-1 Forward Primer: 1 nmol @ 50 µM 20 µl in TE Buffer (10 mM Tris-HCl [pH 7.5] and 1 mM EDTA). Tīmeklis2011. gada 11. nov. · The transposon insertion reaction is made by incubating equimolar concentrations of the target pGLO DNA with EZ-Tn5 transposon as described in the EZ-Tn5 Transposon Tools Technical Manual (EPICENTRE Biotechnologies). Students prepare the transposon insertion reaction mixture by the sequential addition … induction verbe anglais