Phenyl isothiocyanate derivatization
WebAug 4, 2010 · Abstract The applicability of 3-pyridyl isothiocyanate, p - (dimethylamino)phenyl isothiocyanate and m -nitrophenyl isothiocyanate as the … Webcomponents, phenyl isothiocyanate (PITC) is used in pre-column derivatization. Derivatization parameters were optimized and the results are molar ratio of the analytes to PITC, 1:40 and time for vortex processing is 60 seconds. Derivatization result was separated using stationary phase Phenomenex Luna C 18, 5 µm (250 x 4.6 mm). Phosphate
Phenyl isothiocyanate derivatization
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WebJan 15, 2024 · Based on the advantages of isothiocyanate group, we propose 3,5-bis- (trifluoromethyl)phenyl isothiocyanate (BPI) as the derivatizing reagent for determination of the selected biogenic amines by LC-MS/MS method. The derivatization procedure for improved separation of tested BAs in beverages samples was optimized and applied. WebJul 12, 2007 · Before injection samples were derivatized with phenyl isothiocyanate (PITC). The reaction temperature, reaction time, and concentration of PITC used for derivatization were optimized. Chromatographic separation was on a C 18 column with a 65:35 (v/v) mixture of 10 mM phosphate buffer, pH 3.6, and acetonitrile as mobile phase. Heptaminol ...
WebNov 5, 2009 · Benzophenone-4-isothiocyanate was used as a photoactivatable linker for immobilization of antibodies on an aminated Au–thiolate SAM surface [ 20 ]. One of the most widely used isothiocyanates for labeling of amines is the fluorescein derivative. Webby chemical derivatization with 3,5-bis(trifluoromethyl) phenyl isothiocyanate and XPS/NEXAFS analysis Nora Graf & Andreas Lippitz & Thomas Gross & Falko Pippig & Andreas Holländer & Wolfgang E. S. Unger Received: 7 August 2009 /Revised: 10 October 2009 /Accepted: 12 October 2009 /Published online: 5 November 2009 # Springer-Verlag …
WebMar 20, 2001 · Chiral derivatization reagents for drug enantioseparation by high-performance liquid chromatography based upon pre-column derivatization and formation of diastereomers: enantioselectivity and related structure ... 1-phenylethyl isothiocyanate. PAA; phenyl alanine amide. PAME; phenyl alanine methyl ester. PGIT; 2,3,4,6-tetra-O-pivaloyl-β … WebA reversed-phase high-performance liquid-chromatographic method has been used for analysis of the amino acids in edible seaweed. Sample proteins were hydrolysed with hydrochloric acid and the amino acids produced were derivatized with phenyl isothiocyanate. The resulting phenylthiocarbamyl amino acids were chromatographed on …
WebAug 1, 2003 · A reversed-phase high-performance liquid-chromatographic method has been used for analysis of the amino acids in edible seaweed. Sample proteins were hydrolysed with hydrochloric acid and the amino...
WebIn the derivatization reaction vial mix, place 10 µL of xM NaOH and 60 µL of borate. Add 10 µL of the AA sample (in xN HCl). Derivatize with 20 µL of AccQ•Fluor reagent. If there is a … remain boundWebIn organic chemistry, phenylisothiocyanate (PITC) is a compound used as a reagent having molecular formula C₆H₅NCS and the molar mass is 135.18 g/mol. The structure contains … remain challengedWebPhenylisothiocyanate glycosides (see Ref.: 44 for a review) derived from p-amino-aryl glycosides using thiophosgene 45 or thiocarbonyldiimidazole 46,47 have been extensively … remain a matter of speculation meaningWebThe applicability of 3-pyridyl isothiocyanate, p-(dimethylamino)phenyl isothiocyanate and m-nitrophenyl isothiocyanate as the derivatization reagents for amines in high-performance … remain aware of new safety proceduresWebPhenyl isothiocyanate is reacted with an uncharged N-terminal amino group, under mildly alkaline conditions, to form a cyclical phenylthiocarbamoyl derivative. Then, under acidic conditions, this derivative of the terminal amino acid is cleaved as a thiazolinone derivative. remain blessedWebMethods for the quantitative derivatization of amino acids with phenylisothiocyanate and for the separation and quantitation of the resulting phenylthiocarbamyl derivatives by reverse-phase high-performance liquid chromatography are described. Phenylthiocarbamylation of amino acids proceeds smoothly in 5 to 10 min at room temperature. remain afterWebPhenyl isothiocyanate (PITC) is a reagent used in reversed phase HPLC. PITC is less sensitive than o - phthaldehyde (OPA) and cannot be fully automated. PITC can be used … remain calm in a closet ghost exile